FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36570053
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG4823111.3188687476061356No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2062010.5638520677014058No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1711650.46804690165475016No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1517930.4150745966925451No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1435480.3925288267971611No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1400410.38293901296779637No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1275100.3486732709958063No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1185780.32424891481562795No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG857430.23446233452273096No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT729010.199346169938556No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC645930.1766281279384528No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC636000.17391279143073704No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT593580.16231313637964923No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG589150.16110176269090998No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT540530.1478067313711577No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT528720.14457731302713725No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC509530.1393298500278356No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA478090.13073265165899542No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA465410.12726533374179141No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG423320.11575591645984215No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG407740.11149559996535964No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT390020.10665010521040262No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT385150.10531841449614525No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT383640.10490550834038988No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG381460.10430939216850466No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG375170.10258940559916609No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT374800.10248822991861674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)