FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39170697
Sequences flagged as poor quality0
Sequence length41
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG1218750.3111381959835946No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC1190750.303989995378433No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG1087020.27750846506509697No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGG748030.19096673209567855No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG650440.16605270005790299No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG636300.1624428587522964No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTC630390.16093407783884978No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC619170.15806969173921007No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA498010.12713840654916098No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC491790.1255504848433001No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482850.1232681665072235No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGC470920.12022252246366716No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTG466350.1190558340077533No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC441260.11265053567977104No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG440160.11236971351313968No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC434840.11101155539815899No Hit
GGCATGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG432280.11035800562854421No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCC394180.1006313469479494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG183150.030.16041625
GTAACGC183150.029.92875723
TAACGCA198950.027.50109924
CTACGCA43850.026.858451
CGGTAAC207950.026.35176521
ACGGTAA208250.026.31822620
GCGCGGT53350.026.166322
TACGCAT48350.026.0501482
TACGGTG64250.025.1571453
CTACACG53550.025.0978916
CGCGGTG59000.024.5493973
GGTGTCC222550.024.549331
AAGTGGG550250.024.49421333
ATAAGTG552450.024.35626431
CGACCTA19900.024.307331
CAAACGG226600.024.27336117
GGTACGG67000.024.2485731
GAATAAG560300.024.20197329
ACCGCTA87600.024.11328712
CCGCTAC88000.024.08125713
Produced by FastQC (version 0.11.5)