FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12037396
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG203400.16897342249104375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA117850.049.7588655
TCAACGC127200.046.0544784
ACGCAGA129550.045.4611177
GTATCAA136650.045.324411
ATCAACG129750.045.1034743
GGTATCA50500.044.8126531
CGCAGAG136200.042.9810038
AACGCAG137500.042.9182746
GCAGAGT154900.037.6776129
GCAGCGC28750.036.833318
GCTCTAG26000.035.7321931
TGCAGCG32650.032.7968147
GAGTACG153250.024.91146312-13
TATCAAC246900.024.4742162
AGTACGG177850.023.67421712-13
TACGGGT23200.022.8384114
CTAGCCT93650.022.2496994
TGGTCCT70300.021.8488315
CAGAGTA188950.021.54191410-11
AGAGTAC203000.021.29681410-11
Produced by FastQC (version 0.11.5)