FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12263678
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC176750.036.646537
CAGTACG74050.035.891088
GTAGCAA193200.035.4080669
AGTAGCA196800.034.6655358
GTACACC47150.033.258327
AAACGAG164400.029.92630622-23
AGTACGA87650.029.8834349
CGAGAAC154550.029.84471724-25
AAGGCCG160300.029.64220636-37
CAAACGA162750.029.53004320-21
CGAAGTG151500.029.36251640-41
ACGAGAA163550.029.34999824-25
TCAGTCG129600.029.0270584-85
GTAGTAG135650.028.6793966
GCCCCGT29250.028.2798751
GTTCCGT12750.028.1704311
CGCGACA27000.028.13943960-61
CAGTCGG136450.028.05141684-85
GTACGAA55150.027.56260710-11
GCTCTGA135650.027.3495038
Produced by FastQC (version 0.11.5)