FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11852154
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG293740.24783680671040892No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT195740.16515141467112224No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC178430.15054647450581557No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT167890.14165357621914126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA28800.052.0192074
GCGACAT29600.050.989595
CTGCGAC36600.041.6728173
CCGACTC72250.038.7734155
CGACTCC73250.038.3208856
GCTGCGA41100.037.1121332
CCCGACT81100.035.5037044
ACCCGAC84650.033.596673
GAACCCG90250.032.289181
AACCCGA90200.031.8954182
GACTCCC89600.031.14177
ACTCCCT97050.028.9234338
CCGACTG86350.026.380068
GGCTGCG59250.026.2682421
CGACTGA91650.024.8547869
GACCGAC92800.024.5464086
GGTGTGC65800.024.5462287
ACCGACT93100.024.5271367
GTGTGCT99950.023.7935878
TCGATCG60100.023.62127316-17
Produced by FastQC (version 0.11.5)