FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11852154
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165570.13969612612188467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG68400.039.3883558
TAGTAGC180000.038.493627
GTAGCAA192450.037.711879
AGTAGCA197150.036.2852448
GTACACC50350.035.2035147
CGGTACA40700.033.030175
AGTACGA84400.032.3180359
GTAGTAG139900.032.0342946
AAACGAG161750.031.07324422-23
TCAGTCG131700.031.02126184-85
CGAGAAC156550.030.93139624-25
AAGGCCG165000.030.7962336-37
ACGAGAA162650.030.70489724-25
GGTACAC50450.030.642216
GGCGGTA45800.030.5877113
CAAACGA158800.030.5613420-21
GGTAGTA114250.029.9231645
CGAAGTG154550.029.62681840-41
CAGTCGG139350.029.33518884-85
CGATCCT54250.028.6563942-43
Produced by FastQC (version 0.11.5)