FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9557370
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610330.6385961828410954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296930.3106817042763857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258600.27057652889864053No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG143540.15018776085889737No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC101750.10646234267376903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.083.569421
GTATCAA271800.066.781971
GCAGCGC18850.059.3407558
ATCAACG351650.054.0019073
CAACGCA353950.053.718255
TCAACGC359250.052.9091844
TGCAGCG21800.050.7648437
AACGCAG377200.050.4297266
ACGCAGA423900.044.8457
CGCAGAG424850.044.7727288
TATCAAC426000.044.6898542
GCTCTAG21300.043.586681
GCAGAGT460850.041.275249
TGGTCCT38500.031.3782145
AGAGTAC515450.027.78985610-11
CTCGCTC14200.027.2406773
CAGAGTA498950.027.0274610-11
TCTGTCG13700.026.9263618
GTCGTGC10250.026.7080881
TAGCGTG12100.026.5530839
Produced by FastQC (version 0.11.5)