FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9631631
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG137550.1428107036077275No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA98150.10190382085858564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC15150.059.7001088
TGCAGCG18550.049.0757337
CAACGCA79850.047.229325
CGCAGAG84100.044.7869838
ACGCAGA85100.044.3980037
ATCAACG88250.042.9416283
GGTATCA33900.042.8718721
TCAACGC89650.042.3235444
GTATCAA94850.042.137381
AACGCAG97450.038.8914646
GCAGAGT105650.035.602819
GCTCTAG21500.034.630111
TCTGTCG13150.031.6749848
GTCGTGC11700.031.0546261
TGGTCCT38750.027.1705825
GTCTCGC13150.026.271481
TACGGGT22350.026.0771524
GAGTACG107250.024.4364281
AGTACGG131650.023.33087312-13
AATGCGT39400.022.349216
Produced by FastQC (version 0.11.5)