FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9692556
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635150.655296703986028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307120.3168617235742564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269750.27830636211954823No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG144140.14871206315444555No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC103580.10686551617550624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92000.084.409081
GTATCAA282750.066.272981
GCAGCGC19800.057.0951048
ATCAACG368950.053.7716523
CAACGCA372050.053.3553245
TCAACGC375400.052.8950424
AACGCAG392800.050.5174676
TGCAGCG22700.049.8010187
ACGCAGA441150.044.8856477
TATCAAC441600.044.7776222
CGCAGAG443700.044.6276868
GCTCTAG20950.041.4701231
GCAGAGT481650.041.13619
GTCGTGC10400.029.7533931
TGGTCCT38950.029.1795735
AGAGTAC531450.027.8938810-11
CAGAGTA519500.027.1153310-11
CATGCAG43850.026.461725
AGCGTAC5900.026.223331
TCTGTCG13950.026.0175328
Produced by FastQC (version 0.11.5)