FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9692556
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG138820.14322331488206003No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA99070.1022124607791794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC16050.054.856068
CAACGCA79550.046.6869435
TGCAGCG19250.046.3537647
GGTATCA35500.044.117821
CGCAGAG84550.043.482338
ACGCAGA85300.043.4473727
ATCAACG88850.041.5987933
TCAACGC89800.040.9594574
GTATCAA95900.040.735391
AACGCAG95900.038.706496
GTCGTGC9900.035.4897961
GCAGAGT105000.034.734659
TCTGTCG15900.032.1765338
GCTCTAG22000.031.9408151
TGGTCCT37600.026.9099035
TAGCGTG11850.026.610279
ATGTCGG10950.025.5333777
GATGTCG11050.024.7635756
GTCTCGC13950.024.7594181
CCGTATG15000.024.1952639
Produced by FastQC (version 0.11.5)