FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11070722
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG431390.3896674489703562No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA384910.34768283405544825No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA287500.2596939928579184No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG287130.2593597779801534No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG236820.21391558743865127No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA229530.20733065106322787No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG192040.1734665543945553No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG170400.15391950046257147No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC163370.14756941778503696No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA161820.14616932843223776No Hit
GACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTT161460.14584414638900695No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC155880.1408038247189298No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG154810.13983731142377165No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC146880.13267427363816017No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG137710.12439116437030935No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC136240.12306333769378365No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG118570.10710231907187263No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT113120.10217942425073993No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA112420.10154712583334673No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG111540.10075223639433815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA14900.072.601041
TGCTAGT36650.065.3658454
TGCGCAT12250.064.6728062
TGTGCTC174000.061.6114278
GGTGTGC146800.060.3596956
GTGTGCT180500.060.1543127
CTGCGCA12950.056.055211
GTGCTAG42150.054.5865333
TACGCAT55750.054.1194762
CGCATTT91200.053.4206474
CGTGTTC17600.050.6714135
CTACGCA55200.050.4537351
CCGTGCT24150.049.8942034
TGATAGT57050.049.7254834
TTAGTAG77900.048.6525466
CACCGTC55500.048.546679
GTACACC105050.048.4057467
ATTCTCA277350.048.3499267
TGGTGCT46400.047.9566841
TTCACCG119200.047.9528969
Produced by FastQC (version 0.11.5)