FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9258817
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT611300.6602355354901172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296540.32027849778216805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259150.2798953689224012No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG136210.14711382674482065No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC97940.10578025248797983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.082.800641
GTATCAA267300.067.838841
GCAGCGC16300.055.0851178
ATCAACG344700.055.020413
CAACGCA345700.054.8775635
TCAACGC350400.054.17634
AACGCAG368100.051.5815546
TGCAGCG19450.046.4695937
ACGCAGA409100.046.337627
CGCAGAG410650.046.162728
TATCAAC416650.045.5483932
GCTCTAG20800.042.8891941
GCAGAGT444650.042.6863949
TGGTCCT36900.030.6218075
AGAGTAC493600.028.73751410-11
CAGAGTA483000.027.23836510-11
TCTGTCG13300.025.931128
GTCGTGC9200.025.8578321
GAGTACG92100.025.05053312-13
AGTACTT474900.024.29720912-13
Produced by FastQC (version 0.11.5)