FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9258817
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG128430.13871102539341687No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA95470.10311252506664728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC15200.057.1451348
CAACGCA77650.052.8947335
TGCAGCG17850.049.659687
CGCAGAG83400.049.221298
ACGCAGA84550.048.62067
ATCAACG85800.047.6622243
TCAACGC87050.047.114584
GTATCAA91700.046.428521
AACGCAG94650.043.6828236
GGTATCA33150.040.2357981
GCAGAGT103650.039.662599
GCTCTAG20550.032.742721
TGGTCCT38550.026.7132425
TCTGTCG14000.025.9221958
GTCGTGC10850.025.7938561
GAGTACG103250.024.92588412-13
CTAGCCT73000.024.2180084
GTCTCGC13050.024.1831841
AGTACGG126600.024.0417712-13
TATACCG43100.023.7409296
Produced by FastQC (version 0.11.5)