FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004914634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45549884
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG9373042.05775277056688No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT7381991.6206385948205708No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG5741621.2605125404929682No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4724471.037207910342867No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC4514970.9912143793823932No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG3546880.7786803584395516No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA2013420.4420252749710625No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1873650.41134023524626323No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC1872130.4110065351648316No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT1550170.34032358896896425No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1383490.303730740565662No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT1295970.284516641140074No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1286950.2825363946042102No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC1279580.28091838828832144No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC1175160.2579940708520794No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1078740.23682606963389852No Hit
AACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC1056050.2318447177604228No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT1009260.2215724632800382No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA1003320.2202683984881279No Hit
GCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCC934010.20505211385390137No Hit
CATCTTGAGGGGGGCTTCATGCTTAGATGCTTTCAGCACTTATCCCGTCC924520.20296868374022645No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA899770.19753508044060003No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC895740.19665033614575175No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT843160.18510694780254544No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT821800.18041758350032241No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC816910.17934403521203257No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA810800.17800264870048846No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC809570.1777326150819616No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC802300.17613656271879857No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC758260.16646804193837245No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG727660.1597501324042889No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA713430.15662608493141278No Hit
GAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGA709790.155826961052195No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC695800.15275560306586072No Hit
AGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGT689150.15129566520959747No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC687460.15092464340853207No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT658130.1444855490740657No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT657180.14427698652317095No Hit
CCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC642600.14107610021575467No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC639980.14050090665433967No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA633740.13913098000425203No Hit
CAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCC633560.13909146288934568No Hit
CAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGG633440.13906511814607475No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG632070.1387643489937318No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA620360.13619354112954493No Hit
GTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAA618310.13574348509866677No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT616250.13529123367251605No Hit
CCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC614560.13492021187145065No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG606150.13307388444721396No Hit
CACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCCCCCGA605290.1328850804537724No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC599780.13167541765858284No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG586150.1286830939020613No Hit
TAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCG577310.12674236448110382No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT575270.12629450384549826No Hit
TACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCT568290.12476211794524No Hit
CAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCA558250.12255794109157336No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG555590.12197396594906806No Hit
GAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCG539000.11833180519186394No Hit
CAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCC538590.11824179398568832No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG527140.11572806639858842No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT513860.11281258147660705No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA510160.11200028522575381No Hit
TACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCTTA509340.11182026281340254No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG506030.1110935869781798No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC497160.10914627137140459No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT496910.10909138648959019No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT491860.10798271187693914No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT488980.10735043803843716No Hit
TAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTT487940.10712211693008922No Hit
CCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGG484260.10631421146978114No Hit
CCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGG482820.10599807455053015No Hit
CACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTA478790.10511333025568188No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC456080.10012758759166106No Hit
CACCGATGCCTCCACCGAAGCTGGCGCTCCGGTTTCCAAGGCTCCTACCT455640.10003099019966769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)