FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9722913
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG537160.5524681749183604No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT534060.5492798300262484No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT324200.3334391658137844No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC291570.2998792645784242No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG141450.14548109193201667No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC140220.14421603895869478No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA125770.1293542377680434No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC117820.12117767586730438No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA101060.10394004348285334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTC108250.051.8632665
CGACTCC114250.049.0564466
AACCCGA116900.048.805172
GACTCCC117250.047.9228027
GAACCCG122350.046.5580371
ACTCCCT121550.046.3446968
ACCCGAC128400.044.3599933
TTGGACG20350.041.7790837
CCCGACT140250.040.6420334
CTCCCTT146450.038.432759
GCGACAT10100.031.0379375
TCGATCG92600.030.7763616-17
TTCGATC97400.029.52794814-15
CGGAACG92500.029.42304856-57
TCGGCCG97600.029.15186120-21
GGTGTGC53500.028.764817
TAGTAGC76300.028.2619177
CTTTCGA105800.028.17117912-13
AGTAGCA79200.027.5869038
ATCGGCC104350.027.2885518-19
Produced by FastQC (version 0.11.5)