FastQCFastQC Report
Wed 15 Mar 2023
EGAF00004914641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49132263
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2078790.4231008044551092No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1853630.377273483210004No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1224010.24912550842610282No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1005460.20464353534865673No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA999490.20342844782052888No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG883970.1799164023851293No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG835330.17001659378075054No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC737210.1500460094826082No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC714820.14548892242150538No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG693100.14106820196741193No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC681170.1386400622336488No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG664260.13519833189853275No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG571990.11641841125860618No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA560190.11401673071724786No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG544970.11091896988339414No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC537510.10940061930385743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)