FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12723848
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990850.7787345463416413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524930.41255601292942196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473920.37246593954910495No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG312150.24532672820360632No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC197930.1555582870842217No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG174120.13684539456931583No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA162980.12809018152370258No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT162150.1274378631370007No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC153340.12051385712875538No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA151350.1189498648522051No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT142970.1123638069238174No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG141100.11089412573932038No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA141090.11088626648164926No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG139880.1099352963034453No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA139730.1098174074383787No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG139390.10955019267756107No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC137170.1078054374745753No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG136630.10738103756033551No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG136510.10728672646828225No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC135760.10669728214294921No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC134130.10541622314255876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128800.095.049951
GTATCAA422350.069.0771261
GCTCTAG20000.066.6578751
GCAGCGC32500.052.9078258
ATCAACG570200.051.863473
CAACGCA578500.051.263395
TCAACGC579900.051.0267334
TGCAGCG34850.048.99877
AACGCAG612050.048.5398986
ACGCAGA674050.044.02917
CGCAGAG677750.043.779958
CATGCAG40700.042.551845
TATCAAC709750.041.5415152
GCAGAGT718850.041.3099569
TGGTCCT47900.038.3921785
GTCGTGC16350.036.0371861
GTGGTAT38350.034.762911
AGGACCG5400.031.9612715
TGGTATC41150.031.6742272
AGAGTAC799050.030.03030210-11
Produced by FastQC (version 0.11.5)