FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11110976
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA513910.46252462429943153No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA326540.2938895736972162No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG271740.24456897395872335No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG175820.15823992419747823No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC159590.14363274657419833No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG155400.13986170071828072No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA143780.12940357354745433No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC142810.12853056293164525No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA132360.11912544856545455No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG128160.11534540260009561No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG123350.11101634995881551No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG120280.10825331636032695No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC115950.10435626897223071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCT30200.057.0856934
TGCTAGT31050.055.9882354
GTACACC120250.055.2565087
CGACCTA9350.053.498081
TGCGCAT12100.053.0293852
TACGCAT35200.052.738432
TGATCGT37200.051.3945435
CTACGCA34600.051.0808751
CGCATTT57450.050.79654
TTCGACG5800.050.102841
GGTACAC117700.049.294876
TGCTCTG338350.049.088417
TTCAGTA127300.049.0423746
GCGCGGT20900.048.981342
GCTCTGA349100.047.910558
GTGCTAG35950.047.5500533
TGATAGT44150.046.901744
CTCTGAA361700.046.4138079
TACACCT142800.046.38858
CCGGTGC62000.045.267593
Produced by FastQC (version 0.11.5)