FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8909410
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA90750.10185859669719992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC128150.035.9228367
GTAGCAA131100.035.657159
AGTAGCA135850.034.444918
GTACACC37950.033.8553667
GTCCCGT7300.033.774731
CAGTACG48750.032.38458
CGGTACA27850.031.834365
AAGGCCG104450.031.2565136-37
TCAGTCG94050.031.2088984-85
ACGAGAA105700.031.01096324-25
AAACGAG106550.030.89695222-23
CGAAGTG98850.030.40935740-41
GGCGGTA33450.030.3317453
CGAGAAC101750.030.32806224-25
CAGTCGG94550.029.7618884-85
ATCGCGA23900.029.65063718-19
CAAACGA105350.029.33652720-21
GGTACAC36850.029.3335696
CCGGTGC14000.029.1239413
GTAGTAG103600.029.0590556
Produced by FastQC (version 0.11.5)