FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10252742
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC145330.14174744668304343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA33900.058.027434
GCGACAT35050.056.1235355
CTGCGAC41200.048.2016833
GCTGCGA44800.044.539012
CGACATC52950.037.1507076
GGCTGCG59700.033.5869031
CGTCGTA9100.024.50700838-39
ATTCGGC42900.023.2015338-39
TGATTCG43650.022.964536-37
TTCGGCT44350.022.8134940-41
TGATCGC43850.022.69965224-25
TCGACCG10150.022.6646523
TTGATCG45150.022.20156122-23
GACATCT93200.021.8624697
ACGGCGT14000.021.7740718-19
TCGGCTG46500.021.45576940-41
GGTGTGC68850.021.2753167
TCGCCAG47550.021.03278726-27
TACGGCG10800.020.52774218-19
GATCGCC49300.020.4283524-25
Produced by FastQC (version 0.11.5)