FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941925
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC140580.14140118739580113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA32000.065.3467944
GCGACAT33600.062.8971145
CTGCGAC40450.050.009593
GCTGCGA43550.046.774742
CGACATC50050.041.7801676
GGCTGCG58250.034.5934371
TTGATCG40000.025.52173422-23
CGTCGTA7750.024.83099638-39
GACATCT90100.023.6407057
TGATTCG42500.023.2474436-37
TGATCGC43100.023.196324-25
ATTCGGC42500.023.1922238-39
TTCGGCT43850.022.69217340-41
GGTGTGC61800.022.2277167
ACATCTG97400.022.0402958
TCGCCAG46900.021.7173926-27
GTGTGCT94650.021.3879818
TCGACCG9450.021.3565543
TCGGCTG47350.021.1635140-41
GATCGCC48050.021.05087924-25
Produced by FastQC (version 0.11.5)