FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941925
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169690.1706812312504872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGT13900.024.4590851
TCTATCG19800.023.9158213
TATCGGG23950.022.4876545
CCCGTTA11900.022.4363163
CCGATCG19150.022.29632886-87
GCCGTTA9800.022.1037033
CCGTTAT10750.022.0069544
CCCCGTT23350.021.5354162
ATAGCGC19250.021.2515794-95
CGGGGAT43150.021.1889388
TAGTGCG22000.020.90853174-75
TCCGACC14150.020.7706899
ACCCGAT21050.020.28337584-85
TCCGTTA10950.020.2423633
CCGTTAA11950.020.2182664
TCCCGTA16100.019.7912352
CCCGTTT33950.019.5864963
CGGTCGA11000.019.51644538-39
GCCGAAC24450.019.19950966-67
ATCGGGG31550.019.145596
Produced by FastQC (version 0.11.5)