FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004914662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53815593
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2920400.5426679958724974No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2218760.41228942696961457No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1511900.2809408789753557No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1481620.27531425696637774No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1263400.23476467127287812No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1033150.19197967399523036No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1018510.18925927286539423No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC973120.1808249144444065No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT927970.17243515276325208No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC647920.12039633197017824No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT562480.10451989258949539No Hit
GGCAGTATGATGGCCTAGATGCAGAGAAAACAGCTCCTTGGTGAATTGAT559850.10403118664882127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)