FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30234516
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG7805512.5816553504610424No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT3543521.1720114851516064No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2925450.967586185272488No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC2497310.8259798172393433No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2105570.6964126695462894No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1874110.6198577810870198No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1817540.601147377388148No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1615530.5343330119787597No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1134910.37536899879594565No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1094060.3618579506944976No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1093620.3617124216574196No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT1036950.3429689431773937No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT882040.2917327996915843No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG835930.27648201810143086No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC823550.2723873601945538No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA779820.2579237583958678No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG755200.2497807472757295No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC752490.24888442070645353No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT699730.2314341661695527No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT690150.22826560213499036No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT687030.22723366896298258No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT642600.2125385436962179No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT632110.20906899915315327No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC596850.19740683131821923No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC591670.19569355765443708No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC575580.1903718253667431No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG572010.1891910556795419No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT566260.1872892557631814No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC565550.1870544248169873No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG561080.18557598209939924No Hit
GACCCAGGTGCTACACAGAAGTGGATTCAGTGAATCTAGGAAGACAGCAG549180.18164008314206187No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA541940.17924546898650534No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT524080.1733383130723839No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC510590.1688765250946964No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA505200.16709379439049066No Hit
AACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGA479860.15871264484604283No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA472190.1561758091315237No Hit
TACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCC464180.15352651916108068No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC463440.15328176578054037No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG434230.14362062220542904No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT433940.14352470534008216No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG426560.14108378649090994No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC420750.13916214170585697No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC394270.13040393965625247No Hit
CGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCA381890.12630928174937545No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG377300.12479114929440245No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC366120.1210933887613746No Hit
GAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTG357960.11839448661920039No Hit
GTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATCTTTGAGACAAGC351660.11631077540649236No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC342030.11312567398135297No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC339460.11227565210569271No Hit
GAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCC338770.11204743611572945No Hit
AACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC336300.11123048902122329No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA319200.10557470144387296No Hit
CCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAAC318700.10540932753810248No Hit
CCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC316570.10470483469952024No Hit
GGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT305390.10100707416649235No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG304430.10068955626741305No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG302610.1000875952504085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)