FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12908732
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1025860.79470237665481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541430.41942926694891486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488660.3785499613749825No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG319570.24756110824827723No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC206360.15986078260823758No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG178240.13807707836834787No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT168640.13064025188531297No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA165080.12788242873118755No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA156610.12132097869875988No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC156270.1210575910941524No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG145300.1125594674984344No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT143850.11143619683172601No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA143760.11136647658344753No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG143640.11127351625240961No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA142370.11028968608225811No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC142160.11012700550294173No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG141160.1093523360776256No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG140530.10886429433967643No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC140010.10846146623851204No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC139900.10837625260172726No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG139630.10816709185689191No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG134140.10391415671190632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131570.10192325628884387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132550.095.172711
GTATCAA429100.068.9768751
GCTCTAG21500.068.362011
GCAGCGC35850.053.6075448
TGCAGCG37200.051.662117
ATCAACG581650.051.27383
CAACGCA586100.050.9454155
TCAACGC590500.050.5053444
AACGCAG626250.047.7807736
CATGCAG43300.045.0746235
ACGCAGA688450.043.3854267
CGCAGAG693150.043.1169978
TATCAAC723700.041.2349322
GCAGAGT731900.040.842329
GTCGTGC15100.035.860861
TGGTCCT46300.035.0855145
TAGATGG34950.033.8780567
GTGGTAT40500.032.7646941
TGGTATC42700.031.0763422
AGAGTAC821400.029.65909410-11
Produced by FastQC (version 0.11.5)