FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8922277
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG302660.3392183407890161No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA259330.2906545044499291No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA247750.27767575474287565No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG170120.19066881693989102No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA145060.16258181627851276No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG135590.15196793374606055No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG127300.14267658356717686No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG124020.1390003919403085No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA114000.12777007483627778No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC113450.12715364026469925No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG110130.1234326170326252No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG104310.11690961847519417No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC99360.11136170733098737No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC99050.1110142623906431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAGT26350.082.380454
TGATAGT34750.080.218894
GTGTGCT106350.070.492127
TGCGCAT11150.070.162322
TTAGTAG54150.069.109866
GTACACC97800.068.6798257
GTGCTAG31650.067.479593
GGTGTGC86300.067.372436
TGGTGCT32200.067.2182851
TGTGCTC105350.066.852228
AATGCTC14150.066.63255
GGTGCTA33950.064.0278852
GGTGTTA56750.060.6127782
GGTACAC97200.059.3382726
CAATGCT16000.058.56014
CCGTGCT20200.058.4844744
GATAGTA59000.057.016875
CTGCGCA12000.056.674571
TTCAGTA63750.056.6029436
CGCATTT74050.056.4280854
Produced by FastQC (version 0.11.5)