FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12326056
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG316800.25701651850356677No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA185450.1504536406454749No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC185200.1502508182666053No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC169980.13790299184102361No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT167020.13550157487520745No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG166180.13482009168220557No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG153510.12454105352109385No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG150040.1217258789023837No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC139110.11285848449820446No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA137900.11187682418447556No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT133070.10795829582471474No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG130310.10571913676199426No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT129930.10541084674611247No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG129710.10523236305270721No Hit
GTTCTTGGGTGGGTGTGGGTATAATGCTAAGTTGAGATGATATCATTTAC128810.10450220248877662No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG126990.10302565557060589No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA124440.10095686730613587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG22150.067.198061
GGTATCA35800.060.0363961
GCAGCGC28500.055.106628
TGCAGCG30350.051.747577
CAACGCA113300.051.378265
CGCAGAG118600.048.7558028
ACGCAGA119450.048.608077
ATCAACG122000.047.9095543
TCAACGC124550.046.7375144
GTATCAA133800.045.3873181
GCAGAGT130650.044.123659
CATGCAG37150.042.778245
AACGCAG138850.041.773076
TGGTCCT49750.039.361655
GTCGTGC15850.032.679881
GTACACT32300.029.8366586
CTAGCCT110850.028.458414
CTTTCTA79050.028.372439
TAGATGG29750.026.995517
TCTTGGG66750.026.3051763
Produced by FastQC (version 0.11.5)