FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004914688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34340205
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG6907842.011589622135337No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT4238501.234267529853127No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3222240.9383287024640651No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2560810.7457177381439627No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC2220610.64665018744064No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT1836150.5346939542148919No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1771050.515736583401293No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1599940.46590869215836067No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC1483490.43199800350638556No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1299260.37834951771545916No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1076400.3134518270930532No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC1020890.29728710122726404No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC1009940.2940984190397232No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT925630.2695470222149227No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT919590.26778815094435227No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA909440.26483243183900623No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG865760.25211264755117213No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT856130.2493083544492527No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC818400.23832123308524222No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT817970.23819601542856253No Hit
GAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTG805650.23460838396276318No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC782090.2277476211921274No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG775100.2257121062614507No Hit
GAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCC727880.2119614603349048No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG709970.20674599933226956No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA688670.200543357268834No Hit
CCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC684310.19927370847087253No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG658070.19163251937488435No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA641010.18666458164708102No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT639370.18618700732858176No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC635890.18517361792103454No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG621000.1808375925536845No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC615760.17931168436530884No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG583990.17006013796364933No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC577210.1680857758420487No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG575350.16754413667594587No Hit
GGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT564880.16449523233772192No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG558640.16267812029660278No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT550640.16034848947465513No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT511680.1490031873717702No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT508820.14817034435292392No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG504220.14683080663030404No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT499650.14550000502326646No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC488630.14229093856603361No Hit
GGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCC485340.14133287789050764No Hit
GCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATC484240.14101255365248985No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA474990.1383189180146129No Hit
CCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC472320.1375414037277879No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC466770.1359252223450617No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT455650.1326870355025545No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC438810.12778316262235476No Hit
ACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACA434950.12665911575076502No Hit
GAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGA432790.12603011542883916No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA432480.1259398422344887No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC427470.12448091093224399No Hit
GGCTACCTTGTTTCGACTTCACCCCAATCATCTGTCCCACCTTCGGCGGC427020.12434986919850945No Hit
CCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCG419220.12207847914711052No Hit
ACACGTGGAATTCCACTCTCCTCTTCTGCACTCAAGTTCCCCAGTTTCCA416590.12131261301439522No Hit
GGGTTGTTTCCCTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCC412200.12003422810085147No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTCGGCGGC407360.11862480145357315No Hit
CCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAAC395790.1152555728773314No Hit
ACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGAGA386300.11249204831479602No Hit
CGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCA385810.11234935842695172No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC381800.1111816309774505No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC381420.11107097351340797No Hit
GACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACAT377380.10989450994832442No Hit
GCCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGC370490.10788811540292202No Hit
CCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGG368530.10731735585154485No Hit
TACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGCCGCC364220.10606226724622057No Hit
GGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGA363320.10580018377875147No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG357350.10406169677787305No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC355030.10338610383950823No Hit
GGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGG354410.10320555745080728No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC354140.10312693241056657No Hit
TGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTC349390.10174371411003516No Hit
ACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCC348620.1015194871434227No Hit
ACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTC345690.1006662598548844No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT344200.10023236611429664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)