FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34340205
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3675721.0703838256061664No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2110530.6145944673306406No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1886340.5493094755840857No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC1639390.47739668414909No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1532270.4462029274432113No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG1441750.4198431546928738No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC1398790.4073330371790151No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1390600.4049480776250462No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1295780.37733612830791197No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG1258900.3665965302187334No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG1153190.33581337094522296No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG1077400.31374303094579664No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC1049540.305630091608364No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG944310.2749867101841704No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC911960.2655662655479197No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG839290.24440448156905298No Hit
GCGCCGAAAATGTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTT819430.23862117305356798No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG745520.21709829629729932No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC738880.2151647027150828No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG700170.20389220157538374No Hit
GGGCATTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT686870.2000191903338958No Hit
GGGCGCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT637810.18573272931830198No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG599870.17468445514521536No Hit
CGTGCAAGGTTAAGCAGAAGATGCGGAGCCGCAGCGAAAGCGAGTCTGAA591650.17229075947566416No Hit
GGGCCCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT566580.1649902788873858No Hit
GTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATC550720.16037178578287462No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG549430.15999613281283556No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG527900.15372651386326902No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA511810.14904104387262687No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC490120.14272483230662134No Hit
GGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGA483760.14087277580317298No Hit
CCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGT464500.1352641895993341No Hit
GGGTACTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT457260.1331558737054715No Hit
TGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTT454690.1324074798039208No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA441840.1286655102961674No Hit
GTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCCCAAAGTATA440160.12817628782355842No Hit
GGGCAGTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT436130.12700273629700232No Hit
GGGCTCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT435180.12672609263689602No Hit
GGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAT423210.12324038251955689No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC421040.1226084701591036No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA418860.12197364576012287No Hit
AACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCA418720.12193287722073878No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA418280.12180474752553165No Hit
TGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGG417390.12154557609658999No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA413910.12053218668904278No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG412620.12015653371900371No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT408020.11881699599638382No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGG402160.1171105414193072No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG399360.11629517063162552No Hit
GGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAG399040.11620198539874763No Hit
GGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGA382690.11144080240639215No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCT380710.11086421877796013No Hit
CGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTC375410.10932083835841981No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA367640.10705818442260319No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG362160.10546238730956906No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA361660.10531678538319733No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG360270.10491201202788393No Hit
GGGCACTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT359250.1046149840980856No Hit
GAACCGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAAT352020.10250958024275043No Hit
GGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCTAGCCATGG348380.10144959821876426No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG347190.10110306563399955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)