FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7959771
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1138011.4297019348923479No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT825631.0372534586736226No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT582670.7320185467647248No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC386230.48522752727433993No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA312350.3924107866922302No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC299390.376128911246316No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT292520.36749800967892166No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC215220.2703846630763624No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC208840.26236935710838916No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG177490.22298380192093464No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA168630.21185282842936057No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC142440.17894987179907562No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC141770.17810813904068346No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG138330.17378640666923711No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG128280.16116041529335454No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA122540.15394915255727834No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG120350.1511978171231308No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT119880.15060734787470645No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT117040.1470394060331635No Hit
GAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTG116930.1469012111026812No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC115770.14544388274486791No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT111740.1403809230190165No Hit
GAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCC105580.1326420069120079No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG101810.12790568974911465No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC101050.12695088841123697No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT95440.11990294695663982No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG95060.119425546287701No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA93980.1180687233338748No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA90580.11379724366442201No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC89130.11197558321715537No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT88440.11110872410776641No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT86990.10928706366049978No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG86530.10870915758757381No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT84850.10659854410384419No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC82290.1033823711762562No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC80710.10139738944751049No Hit
GGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT79830.10029183000365212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCA78650.063.9459848
TTAGAGC84250.062.622476
TAGAGCC85750.062.3936127
GTAGCAA81700.061.7861379
TAGTTTG28300.058.9155277
ACTCCCT232800.056.0939378
GAACCCG228300.056.089731
CGACTCC234400.055.1413466
AGAGCCA97600.054.765758
CGGCCTA105100.054.7017829
TACACAC107850.054.4549031
ACACACC104700.054.2945822
GAGCCAA99850.053.5781829
CCGGCCT109100.052.781358
CTCCCTT250500.052.1118749
CCGACTC249450.051.8889925
AAAGCGC17850.051.516787
GTGCTAG5250.051.3086363
CACCCGG114950.050.0188185
TTAGTAG11050.050.015716
Produced by FastQC (version 0.11.5)