FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11561710
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1211131.0475353559291836No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1042110.9013459081744828No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT568720.4918995546506529No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC533400.4613504403760344No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC524480.4536353186509608No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA429040.3710869758885148No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA342710.2964180904035822No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG339940.29402225103380036No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG221490.19157200794692134No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC219130.18953078740082566No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT217410.1880431181892644No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG198730.17188633861254088No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC198670.17183444317492827No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT182010.15742480999782904No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG172070.14882746583334128No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT155730.13469460832350924No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT152220.131658725223172No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT147930.12794820143387095No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA146200.12645188298270757No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG143930.12448850559303079No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG134560.11638416808586274No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG124310.10751869749370983No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG116730.10096257387531775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCC198650.056.9300586
CCGACTC203600.055.6161775
ACTCCCT210750.053.9544568
GACTCCC221200.051.2766887
CCCGACT223900.051.0835044
ACCCGAC222500.050.956853
GAACCCG225600.050.2654041
AACCCGA227900.049.6501772
GTAGCAA120100.049.514689
CTCCCTT241000.047.1626639
AGTAGCA129400.045.8457348
TAGTAGC130800.044.9192737
TAGAGCC131200.042.4653747
TTGGACG29800.041.9189387
TTAGAGC137500.040.4506036
GTAGTAG120050.037.8634836
GGTAGTA109950.036.1146745
CGGCCTA52400.034.807489
GCCCTTA164900.034.712812
CCCTTAG163950.034.6787033
Produced by FastQC (version 0.11.5)