FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12872008
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA258160.20055922898742762No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG229810.17853469326619437No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC147350.11447320418073077No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC140970.10951671254399468No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA137000.10643250066345515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC25350.059.141368
GGTATCA52550.057.6801151
GCTCTAG24450.051.8778231
TGCAGCG29100.051.6951527
GTATCAA151200.049.1522981
CAACGCA146350.048.4544535
ATCAACG154800.045.9857523
CGCAGAG153200.045.9403958
ACGCAGA154450.045.8892147
TCAACGC160300.044.2179954
GCAGAGT168050.041.5295229
AACGCAG173800.040.9157186
CATGCAG38350.040.1776545
TGGTCCT43750.036.170445
GTACTGG40250.027.962561
GTCGTGC16200.027.5693971
TAGCGTG20350.027.4827989
GTACTTT139800.026.6228081
CTAGCCT109650.026.1612934
AGGACCG9000.025.779375
Produced by FastQC (version 0.11.5)