FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27308019
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3192941.1692316458400003No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1405560.5147059550529828No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1175800.4305694968206958No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1021410.37403299008983404No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC940600.3444409497444688No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA882020.322989375391895No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA715600.2620475692506293No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT548710.200933652492332No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG482000.17650493065791406No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG412720.1511350933218554No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT373890.13691582681262965No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC348060.12745706673193685No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG341880.12519399521437274No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC336190.12311035816988408No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC325260.11910787084189445No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT314440.11514566472214628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)