FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004914710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291645
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG17880.6130741140770457No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG10830.3713418711104254No Hit
NAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT9490.32539560081606067No Hit
NTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA7070.24241800819489448No Hit
NTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT6540.22424522964563082No Hit
NGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC6540.22424522964563082No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT5100.17487013320989558No Hit
NACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC4720.16184059387268768No Hit
NGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA4680.16046906341613948No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4130.14161051963860172No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA3670.12583791938829741No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC3500.12000891494796757No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)