FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12992624
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260790.2007215786433903No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG225620.17365237383918752No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC149690.11521152309187122No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC143010.11007014441424612No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA138090.10628338047803122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.059.5651051
GCAGCGC24200.058.7679638
TGCAGCG27800.051.366837
CAACGCA142700.051.0222855
GCTCTAG23550.050.3331531
GTATCAA151900.049.56571
CGCAGAG149950.048.374388
ACGCAGA151700.047.850957
ATCAACG152600.047.6827623
TCAACGC158400.045.9582444
GCAGAGT166050.043.510419
AACGCAG168450.043.303276
CATGCAG39300.037.5371745
TGGTCCT44250.037.5053635
GTCGTGC19550.026.5072361
GTACTTT138250.026.4973181
TCTGTCG9450.026.446958
GTACTGG38900.026.3372731
GAGTACG182400.026.04064412-13
TATCAAC298100.025.0478952
Produced by FastQC (version 0.11.5)