FastQCFastQC Report
Wed 15 Mar 2023
EGAF00004914715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46724731
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3187240.6821312679146296No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2007080.42955410487007406No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1560330.3339409273431665No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1383170.29602524624486337No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1376080.294507848531006No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1370120.2932322927659016No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG1079400.2310125659150397No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG1050980.2249301338941898No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC1015020.21723399541882862No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC1013210.21684662026197646No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1005600.21521793244780799No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC999450.21390171299220537No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG995950.21315264500934203No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC852670.18248794198515556No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG825930.17676506259608002No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG801570.17155154943535147No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG743100.159037833733061No Hit
GCGCCGAAAATGTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTT692520.14821273128356802No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG646580.1383806789599281No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG623110.13335764308627052No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG605010.12948389151774892No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA584160.12502158653412043No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA503410.10773951807234589No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA494570.10584758636705688No Hit
GGGCATTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT490530.10498294789540896No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC479980.10272504297563533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)