FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6520613
Sequences flagged as poor quality0
Sequence length101
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1969083.0197774350356323No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1529852.3461751218788787No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1445562.216908134250568No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG1263181.9372105045951968No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1127761.729530643821371No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG388630.5960022470280018No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA355680.5454701881556228No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC246600.37818530251680327No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG239980.3680328827979823No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC236030.3619751701258762No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC219820.3371155441980685No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT197630.3030850013641356No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT192820.2957083942874696No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC185270.2841297282939503No Hit
AACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC172390.2643769841884498No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC166550.2554207710226017No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC163530.250789304625194No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT161640.24789080413145206No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC160280.24580511065447375No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA150680.2310825684640386No Hit
CCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC145360.22292382633350574No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG144740.2219729954837068No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC143730.22042406135742146No Hit
AGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGT141630.21720350525326376No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA139770.21435101270386694No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG136580.20945883462183693No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC134290.2059468948701602No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC133950.2054254715009156No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT123560.18949138677605923No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC121090.18570339935831187No Hit
CCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGG120040.184093121306233No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC118090.18110260492380087No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT116620.17884821565089049No Hit
CACAGGGGGAAACCCCCGACTACCATCGGCGCTGAAGAGCTTAACTTCCG113410.17392536560596372No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA113040.17335793429237406No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA107930.165521247772257No Hit
CAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCC107070.16420235336769717No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG106790.1637729458871428No Hit
CATCTTGAGGGGGGCTTCATGCTTAGATGCTTTCAGCACTTATCCCGTCC102060.15651902666206383No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA99880.15317578270631918No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT98340.1508140415632702No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT97290.14920376351119136No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG96370.14779285321794133No Hit
GAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTG94970.14564581581516953No Hit
GACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACAT94670.14518573637171842No Hit
CAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCA93880.1439741938372972No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT93620.14357545831963958No Hit
CCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC93530.14343743448660426No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC91170.13981814286478894No Hit
TACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCC90280.138453240515884No Hit
GCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCC86200.13219616008494908No Hit
CACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCCCCCGA85280.13078524979169903No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA85220.1306932339030088No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC85080.13047853016273164No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT85070.13046319418128327No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT84770.13000311473783216No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC81390.12481955300828312No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT81240.12458951328655757No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT81100.1243748095462804No Hit
ACAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTC80330.12319393897475589No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG80150.12291789130868525No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT78900.121000893627639No Hit
GATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC77880.11943662351990526No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC74090.11362428655097305No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA73800.11317954308897031No Hit
GTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAA73770.11313353514462521No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG72450.11110918559344037No Hit
GAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGA72180.11069511409433438No Hit
GAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCC71280.10931487576398109No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG70940.1087934523947365No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT70460.10805732528521475No Hit
CAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGG70300.10781194958204084No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC69160.10606364769692667No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT69070.10592562386389133No Hit
CACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGC68670.10531218460595652No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG66460.10192293270586676No Hit
CCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGG65660.10069605418999716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)