FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14522060
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG337610.2324807912926954No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT239890.165190062566881No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC193270.1330871790916716No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC192220.13236414117556325No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT149540.10297437140460788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA64650.041.68344
GCGACAT66150.040.809175
CTGCGAC70550.038.4636573
CCTCGAC31650.037.5358121
GCTGCGA72350.037.4420852
CCGACTC101900.033.817515
CGACTCC103750.033.1239976
GAACCCG106200.032.8078961
CCCGACT114900.031.7891774
ACCCGAC115600.030.2970773
CGCGTCG19800.029.04898812-13
GGTGTGC93450.028.888787
GGCTGCG96500.028.563391
GACTCCC123400.028.002927
AACCCGA126400.027.7828792
CTCGACC42950.027.6554092
ACTCCCT128950.026.7976788
GTCGCAT21050.026.56853114-15
GCGCGGT33300.025.8004116
GTGTGCT129450.025.7511718
Produced by FastQC (version 0.11.5)