FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13109297
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG88300.041.6037568
TTCGACG5400.038.3266871
GTAGCAA210450.037.8523379
TAGTAGC203150.036.692897
AGTAGCA220000.035.756258
AGTACGA107100.034.501259
GTAGTAG145300.032.7905276
TCAGTAC119650.030.6693447
GTACACC58600.030.6279497
AAACGAG183350.030.30927322-23
CGACCTA7550.030.2979871
CGAGAAC173900.030.24283224-25
GTACGAA69500.030.15175810-11
AAGGCCG177150.030.09448236-37
TCAGTCG142400.030.0422984-85
ACGAGAA181900.029.81546424-25
CGATCCT69050.029.74641242-43
CTCACGA70900.029.55022838-39
CAAACGA183900.029.54804820-21
ATACCGG43500.029.4547679
Produced by FastQC (version 0.11.5)