FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12954165
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA261720.20203540714511511No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG230250.17774206210898194No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC148350.1145191527203799No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC141730.1094088271995918No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA137100.10583468714502246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC24150.057.3953448
GGTATCA52450.057.2241741
GCTCTAG21200.051.9673271
TGCAGCG27200.051.1758087
CAACGCA144500.049.9199375
GTATCAA149550.049.8156051
CGCAGAG153400.046.846758
ACGCAGA154100.046.7089927
ATCAACG155050.046.1388663
TCAACGC160850.044.4384
AACGCAG168750.042.723476
GCAGAGT168800.042.261359
CATGCAG39150.037.093465
TGGTCCT44900.036.584915
TAGCGTG21050.029.6779429
GTACTTT137450.026.9488431
AGGACCG10400.026.324725
CTAGCCT112650.025.9407674
GAGTACG184350.025.72708912-13
CTTTCTA81950.025.4832489
Produced by FastQC (version 0.11.5)