FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12746946
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG328960.2580696584107283No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT302610.23739804028353145No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC236740.18572291747372272No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT203610.15973237824966074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCC95800.045.59656
GCGACAT35400.045.1128125
CCGACTC97400.044.7325945
TGCGACA36100.044.5463264
CCCGACT107750.041.623544
GAACCCG108850.040.511891
ACCCGAC111600.039.4035073
GACTCCC115100.037.950867
AACCCGA118200.037.205062
ACTCCCT118650.037.0033158
CTGCGAC42950.035.9494483
GGTGTGC71550.032.8971337
GCTGCGA49550.031.0677742
CTCCCTT152350.029.0008969
GTGTGCT109800.028.63378
GCGCGGT26700.027.7183696
CGGACCA35450.027.4690135
TCGATCG79950.027.09028416-17
CGGAACG76100.026.7259156-57
CACGGAC40650.025.976553
Produced by FastQC (version 0.11.5)