FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12746946
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138070.10831614097996493No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA134340.10538994987505243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC205750.039.5859037
GTAGCAA214900.039.1409539
CAGTACG87200.038.2398268
AGTAGCA219850.037.9512568
GTACACC57500.034.8201457
GTAGTAG150750.033.0527426
AGTACGA104150.032.3049139
AAACGAG187200.031.22578622-23
GGTAGTA126150.031.1792775
ATACCGG46450.031.1320699
TCAGTCG153350.031.05049584-85
AAGGCCG188350.030.84281336-37
CGAGAAC183250.030.81790424-25
CGTCGGT30200.030.805119
ACGAGAA189200.030.68901424-25
CGAAGTG174600.030.65057440-41
CAAACGA185550.030.41678220-21
CAGTCGG159750.029.59985284-85
GGCGGTA52250.029.5563663
TACCGGC29250.029.48642210-11
Produced by FastQC (version 0.11.5)