FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9641728
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1026041.0641660913894273No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT895860.9291487998831744No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC687410.7129531137986884No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT604950.6274290251705918No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG379000.39308306560815653No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG377390.39141324044818526No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA265740.2756144956588694No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC262980.27275193824177574No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC250440.2597459708467196No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA219630.2277911179406845No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT188250.19524508469851048No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC174860.1813575325916682No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA171190.17755116095372114No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG169840.17615099699970793No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT156270.1620767563656639No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG151770.15740954318561984No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA148590.15411137920505535No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT130760.1356188434272363No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG128920.1337104718158405No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC125410.1300700455354061No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA123920.1285246793935693No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG120470.12494648262220216No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA120170.12463533507686589No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC115360.11964660276664099No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT113460.11767600164617795No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC111350.1154875972439795No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT111000.11512459177442053No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG109820.11390074476276453No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG101840.10562422005681968No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA101610.1053856736053952No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC101110.1048670943631681No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG99860.1035706462576003No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT98980.10265794679128057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACG69850.060.043437
ACTCCCT195950.050.2972228
CGACTCC196300.050.2075466
GCTTGGA85450.049.8043335
CCGACTC209650.047.0557635
CTTGGAC91550.046.174576
CTCCCTT217800.045.3603749
GAACCCG220650.044.650671
GACTCCC221050.044.5430537
AGTAGCA58300.044.483128
GTAGCAA58850.044.470949
ACCCGAC235250.042.121873
TGCTTGG102950.041.6179474
CCCGACT245200.041.4372374
AACCCGA244850.040.5285762
ACTGCTT112600.039.3610082
CGGCCTA50600.035.857839
TAGAGCC85500.035.7203757
TTAGAGC85850.035.4640966
TAGTAGC77750.033.966097
Produced by FastQC (version 0.11.5)