FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9641728
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA515570.5347277998300719No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG395300.4099887489047606No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA273950.28412956681623874No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG218020.2261212927807132No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG215290.22328985011815308No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA211570.21943162055598334No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG183190.18999706276717204No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC177550.1841474889148501No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG153990.15971203502110823No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC136580.14165510580675997No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG132340.13725755383267396No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC132300.13721606749329582No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC127680.13242439529511724No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA124370.1289914007115737No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG121340.12584881050367736No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG113290.11749968470382073No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG108050.11206497424528052No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG97960.10160004513713723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA9800.060.523241
TTCGACG5850.060.02251
TGCTAGT34350.059.111494
GTACACC80100.058.867727
TGATAGT49000.055.283424
GATAGTA60300.053.3403665
GGTACAC80250.052.669116
GTGCTAG38400.052.26073
GCGCGGT18400.051.8253332
CCGTGCT22100.051.7344254
CTACGCA24550.051.4124571
GGTGCTA40900.050.805962
TACGCAT25650.050.6788862
GGTGTTA60950.050.5945242
TCGACGT7900.050.444762
TGATCGT33100.050.306345
TTCAGTA116600.049.268456
CGCATTT42250.048.620124
TACACCT98900.047.917328
TAGGCGC6200.047.4398845
Produced by FastQC (version 0.11.5)