FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6955489
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT641030.9216174448697999No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG639400.9192739719666008No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC419720.6034370840066026No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT407740.5862132770248073No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC186240.26775975060847623No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG170100.2445550557264917No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA162250.23326900524175942No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC158820.22833764815097832No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC143040.20565053010651013No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA134270.193041783259236No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG107980.15524429698616446No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG93820.13488627471052No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC92700.133276035660469No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC89450.1286034669884461No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA86510.12437658948206229No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT83900.1206241574100685No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG83060.11941647812253027No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA82000.11789250187873204No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA75420.10843234745968257No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA75090.10795790202529254No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG73650.105887594675227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCC105500.059.6519976
CCGACTC110150.057.306255
TTGGACG22800.053.954347
ACTCCCT117050.053.846948
CCCGACT120200.053.8195954
ACCCGAC118100.053.413033
GACTCCC118300.053.2378277
AACCCGA119050.052.9480362
GAACCCG120300.052.5639461
CGTCAGG25000.045.597294
CTCCCTT140200.045.023429
GCGACAT7700.044.4123045
ACGTCAG27250.042.3583263
TGCGACA8250.042.0278054
CACGTCA28600.040.0275842
GCACGTC29250.039.661211
GTAGCAA47600.035.8219349
TGCGGCG12750.035.7620666
ACGTACG21950.035.487644
CGTACGA21700.035.458215
Produced by FastQC (version 0.11.5)