FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12329290
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229560.1861907701092277No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG215590.17486002843634954No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC144080.11685993272929746No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC134090.10875727637195655No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA132780.10769476587865158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.057.581911
GCAGCGC22050.055.0423858
GCTCTAG21300.051.7209551
TGCAGCG24000.050.568967
GTATCAA145700.050.3938981
CAACGCA143800.049.677635
CGCAGAG150900.046.8779148
ATCAACG154200.046.327133
ACGCAGA153500.046.2765587
TCAACGC159600.044.647774
AACGCAG167300.042.7441946
GCAGAGT167600.042.0300989
TGGTCCT41900.035.2351575
CATGCAG36050.034.5127455
AGGACCG10400.031.4824055
GTCGTGC17400.028.7477871
TAGCGTG18400.028.1309369
GTACTTT134150.026.1012341
TCTGTCG9300.025.5889668
GAGTACG180000.025.34208312-13
Produced by FastQC (version 0.11.5)