FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11953820
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC129090.10799058376318198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA27300.049.5849154
CTGCGAC29950.045.3620723
GCTGCGA34850.039.93922
GCGACAT34300.039.1885955
CGACATC43350.031.116666
GGTGTGC89050.029.1756677
ACGGACC30850.027.7130744
GGCTGCG52050.027.3146631
CGGACCA33850.025.6779165
GTGTGCT132950.025.2580748
CACGGAC36250.024.1120153
TGATCGC30050.022.28743724-25
TTGATCG30700.021.66051722-23
GCGGCGT23750.021.5985136
TGTGCTC116200.021.4596359
ACACGGA42850.020.9529692
CGCGAGT28350.020.60907428-29
CGAGTCG28250.020.26153230-31
GATCGCC33600.020.0740324-25
ATTCGGC34900.019.3946238-39
Produced by FastQC (version 0.11.5)