FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7116361
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1560072.19222998945669No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG912181.2818068110934788No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC789421.109302914790298No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT719161.0105726789295821No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG380760.5350487419061513No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG299170.42039744751566144No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC241990.3400473921994682No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA214370.3012354207438324No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC208120.29245284211972944No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA206570.2902747626209519No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA178450.25076018487538787No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC158820.22317586193280528No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC145900.20502051540105962No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG143160.20117023293225286No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG141400.19869705879170546No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC137520.19324483398186235No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA135320.19015336630617813No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA132810.18662628273073836No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT122890.17268657393856215No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA116770.16408667294984053No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC111620.15684982816357967No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA109920.15446096677782367No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG107010.15037179817044133No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT104440.1467604018402102No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT99000.13911604540579095No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC92400.12984164237873824No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT92160.1295043913595727No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC87700.1232371432534128No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC87650.12316688262441998No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG85660.1203705095905056No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG85040.11949927779099459No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC83330.11709636427944001No Hit
AACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC82180.11548036981260507No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC79220.11132094057622989No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA77740.10924122595804232No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC75400.10595302852117816No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT75350.10588276789218534No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT74950.10532068286024276No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT74660.10491317121208439No Hit
CATCTTGAGGGGGGCTTCATGCTTAGATGCTTTCAGCACTTATCCCGTCC73640.10347985438063077No Hit
CACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTA71840.1009504717368891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCCT235800.069.9979558
CGACTCC237750.069.482316
CCGACTC245900.067.237365
GACTCCC253900.065.156237
CTCCCTT254300.064.961729
GAACCCG255450.064.551371
TTGGACG62500.064.0638057
CCCGACT270500.062.021174
ACCCGAC268550.061.699353
AACCCGA274000.060.439992
GCTTGGA71450.056.304925
AGTAGCA55400.051.956188
GTAGCAA55300.051.878359
CTTGGAC79200.050.495436
TCAGACG11800.048.7044335
GCGACAT7800.046.8879555
AGACGTG12600.046.3660167
TGCGACA7700.046.2654844
TGCTTGG88350.045.5906524
ACTGCTT94150.043.7972642
Produced by FastQC (version 0.11.5)