FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651927
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT284571.7226548146498No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG140410.8499770268298781No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT119070.7207945629558691No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC111050.6722452021184955No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG62980.38125171390745477No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA40130.24292841027478818No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA36280.21962229565834326No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC33060.20012990888822568No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA31920.1932288775472524No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC30470.18445124996443546No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC27000.163445479128315No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC23920.14480058743515906No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA22500.13620456594026248No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG21690.13130120156641306No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA21210.12839550415968745No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA20550.12440017022543974No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC20410.1235526751484781No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA18910.1144723707524606No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG18790.11374594640077922No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT18310.11084024899405362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCCG61950.071.343351
CGACTCC63250.070.518396
ACTCCCT64900.069.150458
CCGACTC65800.067.7155
TTGGACG16200.066.467997
CTCCCTT68700.065.325549
GACTCCC69850.064.2539147
CCCGACT71350.063.03324
AACCCGA70400.062.8948752
ACCCGAC71150.062.688523
GCTTGGA18200.059.4188275
CTTGGAC19550.055.0783276
GTAGCAA14700.054.303079
GAGTAGT9100.053.5680431
AGTAGCA15000.053.2170038
TGCTTGG21350.050.652124
GGACGCG22600.047.4369549
GCGACAT1800.046.412995
TAGTGGT10850.046.199114
TAGTAGC17000.046.1399737
Produced by FastQC (version 0.11.5)