FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10788024
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA135100.1252314603675335No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA119790.1110397974642993No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG117110.10855556124087229No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG112910.1046623552190837No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110100.10205761499974415No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC108080.1001851682940268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC18650.055.189038
CAACGCA59200.047.7323765
CGCAGAG62100.045.0289428
ACGCAGA62650.044.892087
GCTCTAG21550.044.76581
TGCAGCG23300.043.8937037
ATCAACG66400.042.48633
TCAACGC66850.042.198154
GGTATCA28050.040.5489041
AACGCAG77200.036.6619266
TCTGTCG14150.035.3189968
GCAGAGT79700.034.8631179
GTATCAA97150.032.3645631
CATGCAG35450.029.7028565
TGCGGAC2451.8189894E-1129.1422359
GTCTCGC15000.028.980791
TGGTCCT37200.026.0666875
TCTCGCT16300.025.9244442
CTCGCTC14700.025.5071473
TAGCGTG10800.024.7911369
Produced by FastQC (version 0.11.5)